Fluidigm X BioMark Software BioMark HD Software

Each software application was designed to be intuitive and easy-to-use, with the industry’s best graphical user interface. The Fluidigm software features include pipetting templates, easy import/export functionality, predefined experiment layouts, and selection of data viewing options.

Real-Time PCR Analysis
Genotyping Analysis
Digital PCR Analysis



Real-Time PCR Analysis
The Fluidigm Real-Time PCR Analysis software includes:

• Standard curve view - allows you to easily calculate the standard curve by identifying dilutions samples
• Delta delta Ct calculation - you can get delta delta Ct in just three easy steps
• Automated threshold setting - automatically determine the ideal threshold setting for each chip run
• Automatic curve quality score - quickly and efficiently sorts through the real-time PCR curves and exclude those that do not meet your criteria

Genotyping Analysis
The Fluidigm Genotyping Analysis software includes additional features specific to genotyping applications including:

• Automatic cluster calling -  a highly accurate cluster calling algorithm that ranks among the best in the industry and automatically increases in accuracy as it processes more samples
• Multiple chip scoring - combines and scores multiple experiments to decrease the analysis time and improve scoring accuracy for rare genotypes
• Confidence score -  automatically determines the confidence level of cluster calls
• Real time or end point - allows you to view real-time data to aid in assay design while using end point imaging for high throughput applications
   

 
Digital PCR Analysis
The Fluidigm Digital PCR software includes features for digital PCR.

• Multiplex capability – up to four reporter dyes can be used per reaction, allowing you to quantitate up to four targets per sample in each reaction
• Copy number variation – the software will automatically apply statistical correction and determine the ratio of your targets of interest
• End-point analysis -  allows you to use end-point image for analysis, greatly increasing the throughput of the system

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Table and Amplification Curve Views Results may be easily viewed in table format.

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Allele Map Automatic cluster calling.

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Heat Map and Call/No-Call Map The software automatically counts and reports the number of positives for each reporter dye.