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Fluidigm Dynamic Arrays

The 96.96 Dynamic Array.		The 48.48 Dynamic Array.

The Fluidigm Digital Array integrated fluidic circuit (IFC) enables users to conduct digital PCR experiments. The digital array partitions a sample premixed with PCR reagents into hundreds of individual PCR reactions. This partitioning of the mixture allows the absolute quantification of target sequences. Digital PCR is a novel technique for PCR applications demanding extreme accuracy of copy number variation.

Using the Fluidigm Digital PCR technology, there are just five easy steps from setup to analysis to discovery.

Gene Expression and SNP Genotyping Workflow

Prime. Prime the Dynamic Array IFC to close the interface valves, preventing premature mixing of samples and assays.

Transfer. Pipette samples, premixed with master mix, into separate sample inlets and primer-probe sets into separate primer-probe inlets on the frame of the chip.

Load. Place the Dynamic Array IFC on the IFC Controller, and use the software interface to pressure load the assay components into reaction chambers. Assay components are automatically combined on-chip.

Run. Place the Dynamic Array IFC on the BioMark System for thermal cycling and end-point or real-time fluorescence detection.

Analyze. Use the analysis software to view and analyze color-coded allele maps and cluster diagrams for one or more chip runs.

The Advantages of Dynamic Arrays:

*Society of Biomolecular Sciences

See how the competition stacks up against the 96.96 dynamic array:

Comparison of materials and pipetting steps between conventional microplates and dynamic arrays; 96 samples against 96 SNP's

Learn more about Fluidigm's applications for dynamic arrays in Genotyping and Gene Expression.

*TaqMan is a registered trademark of Roche Molecular Systems, Inc.