Mutation Discovery, Gene Scanning and Genotyping
The LightScanner is the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in 96- or 384-well plate system. Standardization is important for research, making the plate format preferable in many settings. It is compatible with nearly all thermal cyclers and qPCR machines.
The LightScanner is unsurpassed in its temperature control for plate-based systems. It also has the highest data quality. With superb sensitivity and specificity, this instrument is designed to meet the needs of high-throughput scanning projects. Simply add LCGreen® Plus dye to the initial PCR reaction– then transfer the PCR plate to the LightScanner for melting. Intuitive analysis software groups the results and provides automatic results.
LightScanner Software
Our comprehensive software package provides both assay design and data analysis modules. The primer design module streamlines assay design for Hi-Res Melting. The analysis software features Call-IT®, a patented scientific method where the software critically analyses each sample, defines and groups wild-types and variants. This software also provides users with simple data management tools. >> More information
| Instrument Specifications |
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| Size (w x d x h) |
7.8” (20 cm) x 16.5” (42 cm) x 31.8” (81 cm) |
| Weight |
25 kg / 55 lbs |
| Voltage Input |
100-240 VAC, +/-10%, 50-60 Hz with automatic detection and configuration, 2.5 A |
| Wattage |
800 W maximum including computer and monitor. |
| Cooling time |
95° to 60 °C (non-linear), ~ 180 seconds at ambient temperature 25 °C |
| Heating rate |
0.1 °C per second |
| Single run time |
15 minutes, including cool down |
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| Sample Handling and Filter System |
| Sample format |
Standard 96- or 384-well trays |
| Reaction volume |
5-100 µl |
| Sample temperature range |
40° to 100 °C |
| Excitation filte |
470 +/- 20 nm |
| Emission filters |
510 and 600 nm |
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| Computer and Software |
| LightScanner Software |
Accurately performs mutation scanning and genotyping by Hi-Res Melting curve analysis. >> read more |
| Computer |
Pentium-based PC with Windows XP Professional, 17”- 1280 x 1024 graphics monitor and software. |
Networking
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Ethernet connection allows export of data in standard spreadsheet format compatible with LIMS software. |
LightScanner Assays for Hi-Res Melting
Idaho Technology has designed and optimized the following assays for use with the LightScanner System. The list continues to grow, based on requests and suggestions from users.
Click on the type of assay below to go to the specific list of LightScanner assays:
Many of the SNP genotyping assays use an unlabeled probe, also known as a LunaProbe, in combination with LCGreen Plus Dye, to genotype samples.
LightScanner customers can obtain the protocols free of charge, by selecting any of the assays listed here and filling in and submitting the form. They will receive a 2 page protocol for the assay they have chosen that will include the primer and probe sequences, reaction set up conditions (with Master Mix), amplification protocol and example results.
To obtain more information about the LightScanner instrument and its capabilities, or to request a demonstration for your organization, please contact us!
