Fluidigm X BioMark Gene Expression BioMark Gene Expression

   96.96 and 48.48 Dynamic Arrays

   The BioMark™ Dynamic Arrays enable qPCR use for both validation and discovery studies
   by radically increasing sample and assay throughput.

Workflow

From setup to analysis: five easy steps to discovery

1. Prime Prime the dynamic array to close the interface valves, preventing premature mixing of samples and assays.
2. Transfer Pipette samples, premixed with master mix, into separate sample inlets and primer-probe sets into separate primer-probe inlets on the frame of the chip.
3. Load. Place the dynamic array on the IFC controller, and use the software interface to pressure load the assay components into reaction chambers. Assay components are automatically combined on-chip.
4. Run. Place the dynamic array on the BioMark Real-Time qPCR System for thermal cycling and end-point or real-time fluorescence detection.
5. Analyze. Use Genotyping Analysis Software to view and to interact with color-coded allele maps and cluster diagrams for the run or for multiple runs.

Applications

• Gene Expression Quantification
  The BioMark 48.48 Dynamic Array maximizes efficiency for gene-expression analysis using real-time qPCR.
• Single Cell Gene Expression
  The BioMarkT 96.96 Dynamic Array overcomes obstacles for measuring relative gene expression within single cells.