MACHEREY-NAGEL 740901 NucleoSpin Tissue XS
| 740901.10 |
NucleoSpin Tissue XS |
10 preps |
| 740901.50 |
NucleoSpin Tissue XS |
50 preps |
| 740901.250 |
NucleoSpin Tissue XS |
250 preps |
Principle
Purification of genomic, bacterial, and viral DNA from smallest samples
 NucleoSpin® Tissue XS is designed for DNA isolation out of Xtra Small samples like microdissected tissue, small blood samples or small amounts of cells. Based on the reliable silica membrane technology NucleoSpin® Tissue XS is overcoming the limitations of conventional kits (large membrane diameter associated with large default elution volumes and a resulting DNA concentration which is too low to be directly used as PCR template) by an innovative column design. A funnel shaped thrust ring is holding a silica membrane of small diameter. It allows the accurate dispensing of small volumes on the membrane and a reduced elution volume of only 5-30 µl. The high DNA yield of NucleoSpin® Tissue XS and the small volume of elution buffer lead to high concentrated DNA, free of impurities and suitable for sensitive downstream applications.
NucleoSpin® Tissue XS is the ideal solution for: microdissected tissue, biopsies (e.g., fine needle aspiration), dried blood spots, buccal swabs, forensic samples, FFPE samples, and other small samples.
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The innovative new thrust ring in a funnel-design allows standard mini prep spin columns to hold a silica membrane of very small diameter. The small diameter of the NucleoSpin® Tissue XS membrane allows efficient elution in only 5–30 μl resulting in highest DNA concentrations ready for all typical downstream applications. |
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Features
| Technology |
Silica-membrane technology |
| Format |
Mini spin columna – XS columns |
| Sample material |
Tissue samples:
Blood samples:
Cultured cells:
Guthrie cards: |
0.025–10 mg
1–30 µl, fresh or frozen
10–10000
spots of 5–30 mm2
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| Fragment size |
200 bp–approx. 50 kbp |
| Typical yield |
100 HeLa cells:
1000 HeLa cells:
10000 HeLa cells:
0.025 mg mouse liver:
0.25 mg mouse liver:
2.5 mg mouse liver: |
0.1–0.5 ng
1–5 ng
10–50 ng
20–100 ng
200–1000 ng
600–3000 ng |
| A260/A280 |
1.7–1.9 |
| Elution volume |
5–30 µl |
| Preparation time |
~40 min/prep (excl. lysis) |
| Binding capacity |
110 µg |
- High sensitivity for DNA isolation from small sample quantities, like biopsy materials, forensic samples, or FACS sorted cells
- Elution in as little as 5 μl resulting in concentrated DNA
- Excellent DNA recovery and purity for reliable results
- DNA ready to use for multiplex PCR and all common downstream applications
- DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections)
- DNA isolation from cells (e.g., bacteria, yeast, cultured cells)
- DNA isolation from clinical samples (e.g., biopsy samples, fine needle aspirates)
- DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs, finger prints)\
- DNA suitable for sensitive applications (e.g., multiplex PCR, real-time PCR)
Kits to be used for research purposes only
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