Product Class: Other

Thermostable 5´ App DNA/RNA Ligase
NEB1 cloned at NEB recombinant 65 No

Product Introduction

Thermostable 5´ App DNA/RNA Ligase ligates a 5´ pre-adenylated 3´-blocked DNA linker to the 3´-OH of RNA and ssDNA.

  • Isolated from a recombinant source
  • Supplied with 10X reaction buffer
  • Tested for the absence of DNases and RNases
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Catalog # Size Concentration
M0319S 10.0 reactions 20 µM
M0319L 50.0 reactions 20 µM

Featured Videos

View Video Library
  • DNA Ligase

    DNA Ligation

  • RNase_Contamination_Anim_720x405

    Avoiding RNase Contamination

Protocols, Manuals & Usage

Protocols

  1. Protocol for ssDNA/RNA Ligation (NEB #M0319)
  2. Protocol for one-step adenylation and ligation of 5´p DNA and 3’OH RNA using Mth RNA Ligase (NEB #M2611) and Thermostable 5’ App DNA/RNA Ligase (NEB #M0319)

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. How can you synthesize an adenylated DNA linker?
  2. Can I use the wild type Mth RNA ligase included in the 5’ DNA adenylation kit instead of the Thermostable 5’AppDNA/RNA ligase for library construction?
  3. How much Thermostable 5’AppDNA/RNA ligase do I need to add to a reaction?
  4. Why do you recommend adding MnCl2 to the DNA ligation but not the RNA reaction?
  5. Does the Thermostable 5’ AppDNA/RNA ligase ligate dsRNA or dsDNA?
  6. Will PEG or increased time enhance the amount of ligated product?
  7. Why is the RNA running as a smear or stuck in the well?

Tech Tips

Which ligase should I use?

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