Random Primer 6Product information
|1.0 A260 units||-||Unavailable in your region|
DescriptionThis mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate nonradioactive nucleotide in the reaction mixture. Use of synthetic d(N)6 primer ensures the presence of virtually all sequence combination of hexamer primers which results in equally labelled DNA of high specific activity (1,2). Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).
Sequence5' d(N6 ) 3' [N=A,C,G,T]
Properties and Usage
- For use in standard Random Priming Reaction, dissolve 33 µg of (NEB #S1230S) in 80 µl of water or 10X labeling buffer.
- 18.6 nmol of S1230 are supplied.
- Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 132
- Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 137
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- Southern, E.M. J. Mol. Biol.. 98
- Smith, G.E. and Summers, M.D. Anal. Biochem.. 109
- Hasse, A. et al. Methods Virol.. 7
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