Product Class: Restriction Endonuclease

XmaI
cloned at NEB recombinant time saver CutSmart_long dil_A 37 65 Heat CpG

 

R0180_XmaI_cutsite

Product Introduction

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

Bioz Badge Exists : True
Catalog # Size Concentration
R0180S 500 units 10,000 units/ml
R0180L 2,500 units 10,000 units/ml
R0180M 2,500 units 50,000 units/ml

Product Information

Description

Product Source

An E. coli strain that carries the XmaI gene from Xanthomonas malvacearum (ATCC 9924).

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
Product Categories:
Restriction Endonucleases T Z,
time-saver-qualified-restriction-enzymes Products
Applications:
Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
Restriction Enzyme Digestion

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg pXba in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X CutSmart® Buffer
Incubate at 37°C

1X CutSmart® Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml BSA
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBuffer™ 1.1: 25%
NEBuffer™ 2.1: 50%
NEBuffer™ 3.1: 10%
CutSmart® Buffer: 100%

Diluent Compatibility

Storage Conditions

10 mM Tris-HCl
50 mM KCL
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Impaired

Product Notes

  1. XmaI is an isoschizomer of SmaI. XmaI produces a 5´ extension whereas SmaI produces blunt-ended fragments.
  2. Impaired by CpG methylation.
  3. star activity may result from a glycerol concentration of >5%

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Double Digest Protocol with Standard Restriction Enzymes
  3. Restriction Digest Protocol

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is XmaI activity sensitive to dam, dcm or mammalian CpG methylation?
  2. How many base pairs should be added at the end of a PCR primer after the XmaI recognition site to guarantee that XmaI will cut properly?
  3. Does XmaI have any isoschizomers/neoschizomers?
  4. Does XmaI exhibit star activity?
  5. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  6. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  7. Why do I see additional DNA bands on my gel after a restriction digest?
  8. Why do I see a DNA smear on an agarose gel after a restriction digest?
  9. Why is my Restriction Enzyme not cutting DNA?
  10. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
  11. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.