Product Class: Other

Taq DNA Polymerase with Standard Taq Buffer
NEBU cloned at NEB recombinant No pcr 5

This is product is available in a glycerol-free format. Contact us for more information.

Product Introduction

Taq DNA Polymerase is the industry standard for PCR

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Hot start version available (NEB #M0495)
 
Catalog # Size Concentration
M0273S 400.0 units 5000 units/ml
M0273L 2000.0 units 5000 units/ml
M0273X 4000.0 units 5000 units/ml
M0273E 20000.0 units 5000 units/ml

Protocols, Manuals & Usage

Protocols

  1. PCR Protocol for Taq DNA Polymerase with Standard Taq Buffer (M0273)
  2. A-Tailing with Taq Polymerase

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What ends will my PCR products have?
  2. How should I determine the appropriate annealing temperature for my reaction?
  3. What should the final primer concentration be in my PCR?
  4. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  5. What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
  6. Is this Taq DNA Polymerase product compatible with other NEB Buffers?
  7. Can Taq/OneTaq® DNA Polymerases be used for nick translation?
  8. How can I optimize my PCR when using Taq DNA Polymerase?

Troubleshooting

Tech Tips

Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?