E. coli K12 KS1000Product information
|1 vial||-||Unavailable in your region|
DescriptionA suspension of E. coli K12 KS1000 which has been grown in M63 or M9 minimal glucose medium and brought to 50% glycerol. This strain is deficient in the Prc protease, a periplasmic protease (formerly known as Tsp, tail-specific protease). The Prc protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and presumably it can degrade proteins expressed in the periplasm as well.
GenotypeF' lacIq lac+ pro+/ ara Δ(lac-pro) Δ(tsp)= Δ(prc)::KanR eda51::Tn10(TetR) gyrA(NalR) rpoB thi-1 argE(am)
Properties and Usage
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
- KS1000 is EcoK r+m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.
- Silber, K.R., Keiler, K.C. and Sauer, R.T. (1992). Proc. Natl. Acad. Sci. USA. 89, 295-299.
The supporting documents available for this product can be downloaded below.