E. coli K12 ER2925Product information
|200 ul||-||Unavailable in your region|
DescriptionA suspension of E. coli ER2925 which has been grown in L-Broth and brought to 50% glycerol.
Genotypeara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Properties and Usage
- Avoid repeated freeze/thaw cycles.
- This strain is both Dam- and Dcm- , so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes (1,2). Suppresses many amber mutations when glutamine is acceptable but not the S100 or S7 mutations of λ, e.g., λgt11. Strain is identical to our previous Dam- Dcm- strain GM2163 except that the activity of nonspecific endonuclease I has been abolished, dramatically improving plasmid prep quality in many cases.
- Woodcock, D. M. et al. (1989). Nucl. Acids Res. 17, 3469-3478.
- Palmer, B. R., and Marinus, M. G. (1994). Gene. 143, 1-12.
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