Product Class: Other

NiCo21(DE3) Competent E. coli

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Product Introduction

Chemically competent E. coli cells suitable for T7 expression with improved purity of target proteins isolated by IMAC.

  • Superior alternative to BL21(DE3) for routine protein expression
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Free of animal products
  • No dry ice surcharge on competent cell shipments
Catalog # Size Concentration
C2529H 20.0 x 0.05 ml

Protocols, Manuals & Usage

Protocols

  1. 5 Minute Transformation Protocol (C2529)
  2. Expression Using NiCo21(DE3) (C2529)
  3. Protocol for Removal of IMAC Contaminating Proteins (C2529)
  4. High Efficiency Transformation Protocol (C2529)

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
  2. What are the solutions/recipes (C2529)?
  3. Why are there no colonies or no growth in liquid culture (C2529)?
  4. Why is the induced protein insoluble (C2529)?
  5. Why is there no protein visible by SDS-PAGE or no activity (C2529)?
  6. What are the strain properties (C2529)? 
  7. Can I store competent cells at -20°C instead of -80°C?
  8. Which kind of transformation tubes should be used?
  9. What volume of DNA can be added into competent cells?
  10. What is the shelf life for this strain (NEB #C2529H)?
  11. Are NEB's competent cells compatible with the “Mix & Go" protocol?
  12. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  13. Is T7 expression subject to catabolite repression in BL21(DE3) and derivative strains?
  14. What expression plasmids can be used with NiCo21(DE3)?

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