NEB® Express Competent E. coli (High Efficiency)Product information
- Transformation efficiency: 0.6-1 x 109 cfu/μg pUC19 DNA
- Enhanced BL21 derivative ideal for Plac, Ptac, Ptrc expression vectors
- Deficient in proteases Lon and OmpT
- Resistant to phage T1 (fhuA2)
- Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
- Free of animal products
- Versatile non-T7 expression strain
- Protease deficient
GenotypefhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|pUC19 Vector||-20||0.05 ng/μl|
|SOC Outgrowth Medium||4||1X|
Advantages and Features
ApplicationsChemically competent E. coli cells suitable for high efficiency transformation and protein expression. Recommended host strain for pMAL Protein Fusion and Purification System.
Properties and Usage
|Antibiotics for Plasmid Selection||Working Concentration|
- Ships on dry ice
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
- Transformation Efficiency:
The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
- CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?
- What are the solutions/recipes (C2523)?
- What are the strain properties (C2523)?
- What is the difference between NEB #C2523H and NEB #C2523I?
- Why are there no colonies or no growth in liquid culture (C2523)?
- Why is induced protein insoluble (C2523)?
- Why is there no protein visible on gel or no activity (C2523)?
- Can I store competent cells at -20°C instead of -80°C?
- Can the NEB Express Competent E.coli (High Efficiency) be used for the expression of constructs containing a T7 promoter?
- What is the optimal heat shock time for this strain (NEB #C2523H and NEB #C2523I)?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C2523H and NEB #C2323I)?
- Are NEB's competent cells compatible with the “Mix & Go" protocol?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?