NucleoSpin SoilProduct information
- Two alternative lysis buffers and a special additive (Enhancer SX) for optimal processing of various soil samples → high yield and high purity
- NucleoSpin® Bead Tubes with ceramic beads for most efficient cell disruption of Gram-positive bacteria, archaea, yeast, fungi, and algae in soil, sludge or sediment
- NucleoSpin® Inhibitor Removal Column to eliminate all PCR inhibitors, such as humic substances – DNA is ready-to-use for PCR without dilution
Isolation of total DNA from diverse soil types
NucleoSpin® Soil for isolation of total DNA from soil includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important as soil is very heterogenous in pH and the amounts of organic or anorganic matter like humic substances or polysaccharides. The silica membrane in NucleoSpin® Soil Columns is optimized for quantitative DNA binding. NucleoSpin® Bead Tubes, containing strong ceramic beads, allow a most efficient lysis of microorganisms in the sample. NucleoSpin® Inhibitor Removal Columns are included for convenient elemination of contaminants.
NucleoSpin® Soil allows processing of up to 500 mg (wet weight) starting material. Depending on the individual sample, typical yields are in the range of 2 to 10 μg DNA. The eluted DNA is ready-to-use (undiluted) for subsequent reactions like PCR, restriction analysis, and others.
|Format||Mini spin columns|
|Sample material||< 500 mg soil, sludge, or sediment|
|Typical yield||2 – 10 µg|
|Elution volume||30 – 100 µl|
|Preparation time||90 min/10 preps|
|Binding capacity||50 µg|