GATA-3 (D13C9) XP® Rabbit mAbProduct information
Product Pathways - Stem Cell and Lineage Markers
GATA-3 (D13C9) XP® Rabbit mAb #5852
|5852S||100 µl (10 western blots)||---||In Stock||---|
|5852T||20 µl (2 western blots)||---||In Stock||---|
|5852||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||48||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
GATA-3 (D13C9) XP® Rabbit mAb recognizes endogenous levels of total GATA-3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.
Western blot analysis of extracts from CEM and SH-SY5Y cells using GATA-3 (D13C9) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GATA-3 (D13C9) XP® Rabbit mAb (left) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded cell pellets, MCF-7 (left) or HUVEC (right), using GATA-3 (D13C9) XP® Rabbit mAb.
Confocal Immunofluorescent analysis of MCF7 (left) and HUVE (right) cells using GATA-3 (D13C9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Flow cytometric analysis of THP-1 cells (blue) and MCF7 cells (green) using GATA-3 (D13C9) XP® Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells and either 5 μl of GATA-3 (D13C9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCL4 promoter primers, SimpleChIP® Human IFN-γ Promoter Primers #13051, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).
GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 null mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).
- Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
- Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
- Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
- Debacker, C. et al. (1999) Mech Dev 85, 183-7.
- Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
- Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
- Amsen, D. et al. (2007) Immunity 27, 89-99.
- Grote, D. et al. (2008) PLoS Genet 4, e1000316.
- Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
- Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
- Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
- Van Esch, H. et al. (2000) Nature 406, 419-22.
- Malu, D.T. et al. (2011) J Immunol 186, 6271-9. Applications: Flow Cytometry.
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- 13051 SimpleChIP® Human IFN-γ Promoter Primers
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- 9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.