Vimentin (D21H3) XP® Rabbit mAbProduct information
Product Pathways - Cytoskeletal Signaling
Vimentin (D21H3) XP® Rabbit mAb #5741
|5741S||100 µl (10 western blots)||---||In Stock||---|
|5741P||40 µl (4 western blots)||---||In Stock||---|
|5741||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||57||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Vimentin (D21H3) XP® Rabbit mAb detects endogenous levels of total vimentin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein.
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb.
Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).
During mitosis, CDK1 phosphorylates vimentin at Ser56. This phosphorylation provides a PLK binding site for vimentin-PLK interaction. PLK further phosphorylates vimentin at Ser82, which might serve as memory phosphorylation site and play a regulatory role in vimentin filament disassembly (8,9). Additionally, studies using various soft-tissue sarcoma cells have shown that phosphorylation of vimentin at Ser39 by Akt1 enhances cell migration and survival, suggesting that vimentin could be a potential target for soft-tissue sarcoma targeted therapy (10,11).
- Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
- Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
- Leader, M. et al. (1987) Histopathology 11, 63-72.
- Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
- Tang, D.D. et al. (2005) Biochem J 388, 773-83.
- Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
- Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
- Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
- Oguri, T. et al. (2006) Genes Cells 11, 531-40.
- Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
- Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.
- Lacher, M.D. et al. (2011) Mol Cancer 10, 91. Applications: IF-IC (In Cells).
- Matsuyama, M. et al. (2013) J Biol Chem , . Applications: IF-IC (In Cells), Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 15019 Animal-Free Blocking Solution (5X)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 14166 Hematoxylin
- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 5425 Normal Goat Serum
- 13614 Phospho-Vimentin (Ser39) Antibody
- 7391 Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb
- 3877 Phospho-Vimentin (Ser56) Antibody
- 12569 Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb
- 3878 Phospho-Vimentin (Ser83) Antibody
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 14746 SignalStain® Citrate Unmasking Solution (10X)
- 8059 SignalStain® DAB Substrate Kit
- 14177 SignalStain® Mounting Medium
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 3390 Vimentin (5G3F10) Mouse mAb
- 3932 Vimentin (R28) Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.