ATR AntibodyProduct information
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - DNA Damage
ATR Antibody #2790
|2790S||100 µl (10 western blots)||---||In Stock||---|
|2790||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||300||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Bovine, Dog.
Specificity / Sensitivity
ATR Antibody detects endogenous levels of total ATR protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human ATR. Antibodies are purified by protein A and peptide affinity chromatography.
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Recent work has shown autophosphorylation of ATR at threonine 1989, a homologous site to ATM Ser1981. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling (6,7).
- Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
- Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
- Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
- Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.
- Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.
- Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
- Liu, S. et al. (2011) Mol Cell 43, 192-202.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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