ATR AntibodyProduct information
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - DNA Damage
ATR Antibody #2790
|2790S||100 µl (10 western blots)||---||In Stock||---|
|2790||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||300||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Bovine, Dog.
Specificity / Sensitivity
ATR Antibody detects endogenous levels of total ATR protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human ATR. Antibodies are purified by protein A and peptide affinity chromatography.
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Recent work has shown autophosphorylation of ATR at threonine 1989. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling (6,7).
- Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
- Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
- Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
- Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.
- Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.
- Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
- Liu, S. et al. (2011) Mol Cell 43, 192-202.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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