Rabbit anti-CD45 Antibody, Affinity Purified

Name: Rabbit anti-CD45 Antibody, Affinity Purified
Brand: Bethyl Laboratories

Product information

Code Name Size Quantity Price

BET A304-376A

CD45 Antibody, Affinity Purified

100 µl (1 mg/ml)

Unavailable in your region

BET A304-376A

CD45 Antibody, Affinity Purified

100 µl (1 mg/ml)

- 1 +

Unavailable in your region

Product Information
Supporting Documents

Product Information
Supporting Documents

CD45 Antibody, Affinity Purified

Detailed information

Reactivity	Human
Applications	ICC-IF, IHC, IP, WB
Target	CD45
Type	Cell Biology Antibody
Antibodytype	Polyclonal
Antigen	Human
Buffer	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Conjugate	Unconjugated
Format	Whole IgG
Host	Rabbit
Subtype	Primary Antibodies

Images

Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. "

Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for IP at 6 µg per reaction. CD45 was also immunoprecipitated by rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012). For blotting immunoprecipitated CD45, A700-012 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 seconds. "

Detection of human CD45 by immunocytochemistry. Samples: A: Acetone-fixed Jurkat cells, B:Formaldehyde-fixed Jurkat cells, C: Acetone-fixed Ramos cells, and D: Formaldehyde-fixed Ramos cells. Antibody: Affinity purified rabbit anti-CD45 (Cat. No.A304-376A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-201D4) used at a dilution of 1:100. "

Detection of human CD45 by immunohistochemistry. Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti-CD45 (Cat. No. A304-376A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB "

Gene and Protein Information

Gene ID:	5788
Gene Name:	protein tyrosine phosphatase, receptor type C
Gene Symbol:	PTPRC
Uniprot ID:	P08575
Protein Name:	Receptor-type tyrosine-protein phosphatase C

MSDS sheets

Immunogen

Between 1254 and 1304

No supporting documents available

This product has no supporting documents available for download. If you feel like supporting documents should be available for this product, please contact us.

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Bethyl Antibody Validation

Validation is a continuous process at Bethyl Laboratories, and they routinely evaluate new lots side-by-side with old lots to ensure lot-to-lot consistency. Learn more

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Rabbit anti-CD45 Antibody, Affinity Purified

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Gene and Protein Information

MSDS sheets

Immunogen

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