Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C. Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.Clin Chem. 2004. 50(7): 1156-64.
4.
Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT. Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye.Clin Chem. 2004. 50(8): 1328-35. Epub 2004 May 27.
5.
Reed GH, Wittwer CT. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem. 2004. 50(10):1748-54. Epub 2004 Aug 12.
6.
Graham, R., Liew, M., Meadows, C., Lyon, E. and Wittwer, C. T. Distinguishing different DNA heterozygotes by high-resolution melting.Clin Chem. 2005. 51(7): 1295-8.
7.
Zhou, L., Wang, L., Palais, R., Pryor, R. and Wittwer, C. T. High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in Solution.Clin Chem. 2005. 51(10): 1770- 7.
8.
Palais, R.A., Liew, M., and Wittwer, C.T. Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping.Anal Biochem. 2005. 346(1): 167-75. Epub 2005 Aug 31.
9.
Chou LS, Meadows C, Wittwer CT, Lyon E. Unlabeled oligonucleotide probes modified with locked nucleic acids for genotyping by melting analysis.BioTechnqiues. 2005. 39(5): 644, 646, 648.
10.
Margraf RL, Mao R, Wittwer CT. Masking selected sequence variation by incorporating mismatches into melting analysis probes.Human Mut. 2006. Jan 12 [Epub ahead of print].
11.
Vandersteen JG, Bayrak-Toydemir P, Palais RA, Wittwer CT. Identifying Common Genetic Variants by High-Resolution Melting.Clin Chem. 2007. May 24th [Epub ahead of print].
12.
Reed GH, Kent JO, Wittwer CT. High-resolution DNA melting analysis for simple and efficient molecular diagnostics.Pharmacogenomics. 2007. 8(6): in press.
Applications
1.
McKinney JT, Longo N, Hahn SH, Matern D, Rinaldo P, Strauss AW, Dobrowolski SF. Rapid, comprehensive screening of the human medium chain acyl-CoA dehydrogenase gene.Mol Genet Metab. 2004. 82(2):112-20.
2.
Willmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ.Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis.Am J Clin Pathol. 2004. 122(2):206-16.
3.
Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer CT. High-resolution DNA melting curve analysis to establish HLA genotypic identity.Tissue Antigens. 2004. 64(2): 156-64.
4.
Vaughn CP, Elenitoba-Johnson KS. High-resolution melting analysis for detection of internal tandem duplications. J Mol Diagn. 2004. 6(3): 211-6.
5.
Odell ID, Cloud JL Seipp M, and Wittwer CT. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting of hsp65 PCR products.Am J Clin Path. 2005. 123(1): 96-101.
6.
Dobrowolski SF, McKinney JT, Amat di San Filippo C, Giak Sim K, Wilcken B, Longo N. Validation of dyebinding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene.Hum Mutat. 2005. 25(3): 306-13.
7.
Willmore-Payne, C., Holden, J. A., Tripp, S. and Layfield, L. J. Human malignant melanoma: Detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis.Hum Pathol. 2005. 36(5): 486-93.
8.
Chou, L.-S., Lyon, E. and Wittwer, C. T. A comparison of high-resolution melting analysis to denaturing high performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model.Am J Clin Path.2005. 124(3) 330-8.
9.
Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT. Mutation scanning of the RET protooncogene using high resolution melting analysis. Clin Chem.2006 52: 138-41.
10.
Liew M, Nelson L, Margraf R, Mitchell S, Erali M, Mao R, Lyon E, Wittwer CT. Genotyping of human platelet antigens 1-6 and 15 by high-resolution amplicon melting and conventional hybridization probes.J Mol Diag. 2006 8: 97-104.
11.
Liew M, Seipp M, Durtschi J, Margraf RL, Dames S, Erali M, Voelkerding K, Wittwer C. Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting. Am J Clin Pathol.2007. 127(3): 341-8.
12.
Uemura A, Mori S, Sugahara K, Akamatsu N, Tsuruda K, Tsukasaki K, Hirakata Y, Atogami S, Hasegawa H, Yamada Y, Kamihira S. Rapid and high-resolution detection of IgH gene rearrangements using PCR and melting curve analysis.Int J Lab Hematol. 2007. 29(3): 200-7.
13.
Montgomery J, Wittwer CT, Palais R, Zhou L. Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis.Nat Protoc. 2007. 2(1): 59-66.
14.
Seipp MT, Durtschi JD, Liew MA, Williams J, Damjanovich K, Pont-Kingdon G, Lyon E, Voelkerding KV, Wittwer CT. Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.J Mol Diagn. 2007. Apr 26; [Epub ahead of print].
15.
Dames S, Margraf RL, Pattison DC, Wittwer CT, Voelkerding KV. Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays. J Mol Diagn. 2007. Apr 26[Epub ahead of print].
16.
Willmore-Payne C, Holden JA, Layfield LJ. Detection of epidermal growth factor receptor and human epidermal growth factor receptor 2 activating mutations in lung adenocarcinoma by high-resolution melting amplicon analysis: correlation with gene copy number, protein expression, and hormone receptor expression.Hum Pathol. 2006. 37(6): 755-63.
17.
Singer R, Bellfoy D, Desjardins M, Chan F. High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi.Diagn Microbiol Infect Dis. 2007. 57(4): 455-8.
18.
Fortini D, Ciammaruconi A, De Santis Riccardo, Antonio F, Raffaele B, Florigio D, Cassone A, Carattoli A. Optimization of High-Resolution Melting Analysis for Low-Cost and Rapid Screening of Allelic Variants of Bacillus anthracis by Multiple-Locus Variable-Number Tandem Repeat Analysis.Clin Chem. 2007. May 24th [Epub ahead of print].
19.
Hill CE, Duncan A, Wirth D, Nolte FS. Detection and Identification of Cytochrome P-450 2C9 Alleles *1, *2, and *3 by High-Resolution Melting Curve Analysis of PCR Amplicons. Am J Clin Pathol.2006. 125: 584-591.
20.
Kennerson ML, Warburton T, Nelis E, Brewer m, Polly P, De Jonghe P, Timmerman V, Nicholson GA. Mutation Scanning the GJB1 Gene with High-Resolution Melting Analysis: Implications for Mutation Scanning of Genes for Charcot-Marie-Tooth Disease.Clin Chem. 2007 53(2): 349-352.
21.
Lonie L, Porter DE, Fraser M, Cole T, Wise C, Yates L, Wakeling E, Blair E, Morava E, Monaco AP, Ragoussis J. Determination of the Mutation Spectrum of the EXT1/EXT2 Genes in British Caucasian Patients With Multiple Osteochondromas, and Exclusion of Six Candidate Genes in EXT Negative Cases.Human Mutation. 2006. 27 (11): 1160-1172.
22.
Nomoto K, Tsuta K, Takano T, Fukui T, Yokozawa K, Sakamoto H, Yoshida T, Maeshima AM, Shibata T, Furut K, Ohe Y, Matsuno Y. Detection of EGFR Mutations in Archived Cytologic Specimens of Non– Small Cell Lung Cancer Using High-Resolution Melting Analysis.Am J Clin Pathol. 2006. 126:608- 615.
23.
Willmore-Payne C, Holden JA, Layfield LJ. Detection of EGFR- and HER2-activating mutations in squamous cell carcinoma involving the head and neck.Modern Pathology 2006. 19: 634–640.
24.
Willmore-Payne C, Layfield LJ, Holden JA. c-KIT Mutation Analysis for Diagnosis of Gastrointestinal Stromal Tumors in Fine Needle Aspiration Specimens.Cancer. 2005. 105(3): 165-170.
25.
Dobrowolski SF, Ellingson C, Coyne T, Grey J, Martin R, Naylor EW, Koch R, Levy HL. Mutations in the phenylalanine hydroxylase gene identified in 95 patients with phenylketonuria using novel systems of mutation scanning and specific genotyping based upon thermal melt profiles.Molecular Genetics and Metabolism. 2007. 91: 218–227.
26.
Dobrowolski SF, Ellingson C, Caldovic L, Tuchman M. Streamlined Assessment of Gene Variants by High Resolution Melt Profiling Utilizing the Ornithine Transcarbamylase Gene as a Model System 2.Human Mutation 2007.in press.
Reviews & Text Book Chapters
1.
Highsmith WE Jr. SNPs for sale. Cheap! Clin Chem. 2004. Aug; 50(8):1296-8.
2.
Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT.Fifty Years of Molecular (DNA/RNA) Diagnostics.Clin Chem. 2005. Mar; 51(3):661-71.
3.
Wittwer, C. T. and Kusukawa, N. Nucleic acid techniques. In: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th edition (Eds, Burtis, C., Ashwood, E. and Bruns, D.). 2005. 1407-1449. Elsevier, New York.
4.
Wittwer, C.T. and Kusukawa, N. Molecular Diagnostics Technology. In: Clinical Diagnostic Technology, The Total Testing Process Vol 2: The Analytical Phase (Eds, Ward-Cook, K.M., Lehmann, C.A., Schoeff, L.E., and Williams, R.H.). 2005. 341 – 369. AACC Press, Washington, DC.
