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The measurement of contractility of Bronchial smooth muscle cells (BSMCs) and airway fibroblasts are important to study the pathogenesis of allergy/asthma and the mechanism of action of anti-allergic/asthmatic drugs. The conventional gel contraction assay is frequently used to study both fibroblasts and smooth muscle cells in vitro, but it is extremely laborious and low throughput.
The major benefits of this new impedance-based method compared to the conventional collagen gel contraction assay are
The study was published in the October issue of Journal of Pharmacological and Toxicological Methods. (J Pharmacol Toxicol Methods. 2017 Oct 19; 89:47-53.)
Endothelin-1 induced BSMCs contraction was recorded in real time by xCELLigence. Figures adapted from J Pharmacol Toxicol Methods. 2017 Oct 19;89:47-53.