BIOKÉ Webshop

Forgot password?Login

No need to login to view prices!

No account?

Enjoy the advantages of a BIOKÉ webshop account.

Invalid login

Your credentials are incorrect or you are trying to login with a non-existing webshop account. Our website shows product prices without login! Therefore webshop accounts that were only used to view prices have been deleted.

Please create a new account with your shipping and billing details if you would like to be able to order or view your discount.

Webshop under construction

Due to technical maintenance the BIOKÉ webshop is closed until November 15th, 2020. We apologize for any inconvenience caused.

Forgot your password?

Don't worry. Enter your username and we will send you a link to reset your password if an associated user is found.

Need help?

Have trouble resetting your password? Contact us and we will help you.

You've got mail

You will receive an e-mail containing a link to start the recovery procedure within minutes.

Change password

Change your password to something secure.


Your password has been changed.

Password sent

You will receive an e-mail from us containing your new password in the coming minutes.

Please change your password to something memorable and secure upon logging in.


Sign out

Viral Vaccine Development Gets Major Boost From xCELLigence® RTCA


Using xCELLigence Real-Time Cell Analysis (RTCA), Sanofi Pasteur scientists have developed an assay for accurate viral titer determination that is 5-times less labor intensive and 3.5-times cheaper than conventional methods.

Accurate determination of viral titers is critical to vaccine development. Historically, titers have been determined using endpoint assays which are labor-intensive, expensive, and time consuming. This is increasingly problematic in modern production facilities where bioprocess optimization is performed using high-throughput cell culture reactors that can produce batches of virus faster than they can be analyzed. Looking for a solution to this bottleneck, Sanofi scientist David Gaillac and his coworkers have just described a transformative new approach for viral titer determination using xCELLigence RTCA instruments. On the basis of their findings the authors reported having made a major change in their workflow, stating that “The RTCA assay presented here is now being used for titration of viruses to support our bioprocess studies.” To view their complete comparative analysis, which was just published in the Journal of Virological Methods.


Read full publication


Figure: Viral titer determination using xCELLigence RTCA. (A) Monitoring the viral cytopathic effect that known quantities of yellow fever dengue virus induces in Vero cells. Note that the rate and extent of cytopathic effect correlates with the amount of virus used for infection. (B) Production of a standard curve which can be used for determining the concentration of virus in samples with unknown titer. This curve is produced by plotting the CITmed, the time required for the Cell Index to decrease to its median value, as a function of the known virus titer.

Product has been added to your cart.