LightScanner^TM Instrument

Hi-Res Melting

Hi-Res Melting is a post-PCR technique for homogeneous mutation scanning and genotyping. Through innovative engineering and imaginative development, Idaho Technology has perfected Hi-Res Melting, enabling you to detect a single-base change in your sample by just melting the PCR product. Results are fast and obtained immediately after PCR by just transferring the PCR plate or tube to our instrument. With robust data acquisition, unsurpassed temperature control, and the perfect saturation dye, Hi-Res Melting is the most simple and cost-effective solution for re-sequencing and mutation detection projects.

Most re-sequencing efforts end up producing wild type (or normal) sequences. Hi-Res Melting can reduce the amount of DNA sequencing by quickly differentiating and ruling out specimens that do not have sequence variants. Further, with the use of unlabeled probe technology Hi-Res Melting can determine common or known sequence variants, finally identifying samples with unknown variants which truly need to be sequenced.

Mutations in PCR products are detected by changes in the shape of the melting curve compared to a reference sample. Below are examples of SNPs identified in two exons using the LightScanner™ instrument and our LCGreen® Plus Dye. Superior reproducibility is demonstrated by the overlapping curves of duplicate samples for both the wild type and the mutant samples.