Product Class: Other

NEB® 10-beta Electrocompetent E. coli

 Supplied with outgrowth medium optimized for NEB 10-beta & NEB Stable Competent E.coli ; please do not use SOC outgrowth medium.

Product Introduction

NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.

  • High efficiency strain ideal for cloning large plasmids and BACs
  • Available in chemically-competent format (single-use and 200 μl vials)
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
  • Refer to our Electroporation Tips
Catalog # Size Concentration
C3020K 6.0 x 0.1 ml

Protocols, Manuals & Usage

Protocols

  1. Electroporation Protocol (C3020)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. How should I calculate the Electrotransformation efficiency (C3020)?
  2. What are the strain properties (C3020)?
  3. What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly?
  4. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
  5. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  6. How should I store the NEB 10-beta/Stable Outgrowth Medium?
  7. How should fragments be prepared for assembly using NEBuilder HiFi?

Other Products You May Be Interested In