Product Class: Kit

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (NEB #E7103)

Product Introduction

Even more from less, for DNA

Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext® Ultra™ II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.

  • Get more of what you need, with the highest library yields
  • Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
  • Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
  • Improve yields and quality for target enrichment applications
  • Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats

Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available with and without sample purification beads.

Catalog # Size Concentration
E7645S 24.0 reactions
E7645L 96.0 reactions

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. For what type of sample prep/library construction can I use the NEBNext Ultra II DNA Library Prep kit for Illumina?
  2. What type and how much starting material do I need to use when preparing libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina?
  3. Is size selection required and what is the recommended method?
  4. Can I use the NEBNext Ultra II DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the Ion Torrent PGM or Proton?
  5. Are these reagents available in a customized format, larger format or 96 well formats?
  6. How do I know the concentration of my index primers? 
  7. Which NEBNext Oligos can be used with this library prep kit?
  8. Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?
  9. What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer?

Troubleshooting