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NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´ and 3´ restriction enzyme mismatches. Available with (NEB #E5520) and without (NEB #E2621) competentE. coli, this flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning. Request a free sample
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Benefits over Gibson Assembly® Master Mix
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Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over Gibson Assembly.