Save up to 62% with NEB’s new alternative to SuperScript® II. NEB introduced the new M-MuLV Reverse Transcriptase (RNase H-) recently. Same results as SuperScript® II, better price!

M-MuLV Reverse Transcriptase (RNase H-) is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length.

Price Comparison M-MuLV with SuperScript® II

Why M-MuLV Reverse Transcriptase (RNase H^-)

• Value pricing - Save up to 62%, same results as SuperScript^® II - see graph
• High cDNA yield - High quality cDNA even with very low amounts of starting RNA
• Superior performance for longer templates - No worries about long templates
 

Figure 1: Robust cDNA synthesis is achieved even with longer templates Jurkat total RNA (1 μg) was used in a 20 μl first strand cDNA synthesis with 200 units of NEB M-MuLV Reverse Transcriptase (RNase H–). Reactions were incubated at 42°C for 50 minutes, followed by heat inactivation for 5 minutes at 80°C. 1 μl of cDNA was used in a 25 μl PCR using LongAmp Taq Master Mix (NEB #M0533) for 35–40 cycles. Sizes are indicated above gel. Ladder L is the 2-Log DNA Ladder (NEB #N0469).

Figure 2: Generate high quality cDNA even with very low amounts of starting RNA Decreasing amounts of Jurkat total RNA (1 μg – 1 pg) were used in 20 μl first strand cDNA synthesis with 200 units of NEB M-MuLV Reverse Transcriptase (RNase H–). Reactions were incubated at 42°C for 50 minutes, followed by heat inactivation for 5 minutes at 80°C. 1 μl of cDNA was used in a 25 μl PCR using LongAmp Taq Master Mix (NEB #M0533) for 40 cycles. The target is a 0.6 kb fragment of GAPDH. Ladder L is the 2-Log DNA Ladder (NEB #N0469).

Do you like cake?

Order M-MuLV Reverse Transcriptase (Rnase H-) now with the 20% introductory discount. Do you want to test first? Request a free sample*.

Send your first results to info@bioke.com before September 30th, 2012. Ten delicious BIOKÉ cakes are available for the first 10 entries that we receive*.

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*This promotion is valid for orders that are received by BIOKÉ before September 15th, 2012 and cannot be combined with other discounts or promotions. Samples are available as long as stock lasts. Only 1 sample per department. Cake is only provided for the first results, only 1 cake per department. Promotion is valid for customers from the BeNeLux countries only. BIOKÉ cannot be held liable for incorrectly stated prices as a result of entry or printing errors. BIOKÉ reserves the right to adjust the promotional conditions at any time.
SuperScript^® II is a registered trademark of Life Technologies Inc.