Highest Buffer Compatibility
New England Biolabs provides over 170 restriction enzymes that exhibit 100% activity in NEBuffer 4. That is more than just “added convenience”.

In addition to reduced star activity, HF™ restriction enzymes work optimally in NEBuffer 4, which has the highest level of enzyme compatibility and will simplify double digest reactions.

Of the top 20 most commonly used restriction enzymes, NEB provides 18 enzymes with NEBuffer 4, the other 2 are provided with NEBuffer 3. This unrivaled buffer compatibility results in onvenience in reaction set-up and great savings to your budget.

*Applies to conventional restriction enzymes only

As you can see in the example below, EcoRV-HF/KpnI-HF both
exhibit 100% activities in NEBuffer 4, i.e. each enzyme works at regular 1-fold concentration to obtain 100% cleavage.

**Double digest recommendations for EcoRV-HF™ and KpnI-HF™: Digest in NEBuffer 4 at 37°C.

Thermo Scientific Fermentas
Double digest recommendation: The first digestion should be performed in 1X Tango™ buffer (low salt concentration buffer) with 4-fold excess of KpnI. Incubate at 37°C for 1 hour. When the first digestion is complete, add 10X concentrated Tango™ buffer (amount "V") to a final 2X concentration (high salt concentration buffer) and Eco32I (EcoRV).

Many conventional restriction enzymes from Thermo Scientific Fermentas require more units to cleave in double digests! Here: 4-fold excess i.e. 4-times as expensive!

Relative costs of the Top20 most commonly used enzymes

* Average discount from list price needed to match New England Biolabs pricing. Price comparison (€/unit) is based on small pack price list published in February 2012 by the suppliers on their website.

Supplier Enzyme   1. R3136 BamHI-HF™   2. R0144 BglII   3. R0176 DpnI   4. R3101 EcoRI-HF™   5. R3195 EcoRV-HF™   6. R0103 HincII   7. R3104 HindIII-HF™   8. R3142 KpnI-HF™   9. R3193 NcoI-HF™   10. R3131 NheI-HF™

Supplier Enzyme   11. R3189 NotI-HF™   12. R3140 PstI-HF™   13. R3150 PvuI-HF™   14. R3156 SacI-HF™   15. R3138 SalI-HF™   16. R0141 SmaI   17. R0133 SpeI   18. R3182 SphI-HF™   19. R0145 XbaI   20. R0146 XhoI

Restriction enzyme digests are now even easier! The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format; simply add your DNA and digest.